|
KMID : 0900919990230040323
|
|
Korean journal of Animal Reproduction
1999 Volume.23 No. 4 p.323 ~ p.331
|
|
|
Effect of Insulin-like Growth Factor-I and Cumulus Cells on In Vitro Maturation in Porcine Oocytes
|
|
Park C.-K.
Cho J.-W.
Shin M.-K.
Cheong H.-T.
Yang B.-K.
Kim C.-I.
|
|
|
Abstract
|
|
|
|
The effects of insulin-like growth factor-I (IGF-I) and cumulus cells during in vitro maturation in porcine oocytes were examined. When follicular oocytes were cultured in medium with different concentrations of IGF-I, maturation rates were 60, 61 and 62 and 72% for 0, 15 and l0ng/mell IGF-I. In medium with 10ng/mell IGF-I, maturation rates were not significantly difference between oocytes with (68%) and without (52%) cumulus cells during the culture. In medium with-out IGF-I, however, the maturation rates in oocytes with cumulus cells (63%) was significantly (P＜0.05) higher than oocytes without cumulus cells (32%). On the other hand, when IGF-I was added for first 24 h period or later 24 h period of culture, maturation rates were higher in oocytes with (61 and 49%) that than without (49 and 45%) cumulus cells. In experiment used medium without fetal calf serum (FCS) and porcine follicular fluid (PFF), the maturation rates in oocytes with cumulus cells for 48 h (48 and 67%) or first 24 h (46 and 63%) period after culture were significantly (P＜0.01) higher than in oocytes without cumulus cells (16 and 18%) in the presence or absence of IGF-I. These results indicated that cumulus cells is essential on maturation in vitro in porcine oocytes, but IGF-I can promote oocytes maturation of oocytes without cumulus cells in medium with FCS and PFF.
|
|
|
KEYWORD
|
|
|
IGF-I, Cumulus cells, In vitro maturation, Porcine oocytes
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|
|
|