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KMID : 0900920000240040451
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Korean journal of Animal Reproduction
2000 Volume.24 No. 4 p.451 ~ p.455
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Simple Methods for Production of Chimeric Mouse by Coculture with TT2 Embryonic Stem Cells
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Cho Y.-Y.
Moon S.-J.
Kang M.-J.
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Abstract
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Gene targeting are very useful tools for the research on the gene function in vivo, mass production of foreign materials and biomedical approach of therapeutic process. But this process is very complicated and necessary highly skilled technique, because it is very different from ES cell origin, genetic background of embryo, and experimental conditions. We investigated the productivity ability of chimeric mouse after aggregation with TT2 ES cells. Increse of ES cell density caused gradual decrease in embryo development in vitro and in th varepsilon production of chimeric mice in vivo. One million ES cell density for the aggregation was very efficient to produce high percentage chimeric mice in their coat color. These results suggested that appropriate cell density plays a key role in the development and production of chimeric mice by a 8-cell aggregation method.
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KEYWORD
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Gene targeting, Embryonic stem cells, Chimeric mice, Aggregation method
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