|
KMID : 0900920010250040371
|
|
Korean journal of Animal Reproduction
2001 Volume.25 No. 4 p.371 ~ p.379
|
|
|
Development of In Vitro Produced Bovine Embryos after Vitrification with Various Containers
|
|
Lee Y.-J.
Ko D.-H.
Lee H.-T.
Chung K.-S.
|
|
|
Abstract
|
|
|
|
The objective of this study was to establish an effective cryopreservation method of in vitro-produced bovine embryos. For the vitrification, in virtro-produced embryos at 8-cell, morula and blastocyst stages were exposed to freezing solution containing 5.5 M EG (EG 5.5) for 20 sec, loaded on each containers such as EM grid, OPS and Cryo-loop, and then immediately plunged into liquid nitrogen at -196^{circ}C. Thawed embryos were serially diluted in 0.5, 0.25 and 0.125 M sucrose in m-HPBS, each for 1 min, and cultured in CRlaa medium supplemented with 10% FBS. Significant differences in the rates of re-expanded and hatched embryos were not observed among these embryo containers. The total cell number of expanded blastocyst cultured in vitro after vitrification was examined by Hoechst staining. There were no differences between non-vitrified (180.0 pm 5.4) and vitrified groups (178.0 pm 7.5). In addition, when the cellular injuries after vitrification were compared by double staining. There were no significant difference in the ratio of live and dead cells between non-vitrified group (176 : 4) and vitrified group (172 : 6). Therefore, these results suggest that bovine embryos can be cryopreserved easily, effectively and successfully by vitrification using various containers, such as EM grid, OPS or Cryo-loop in the presence of EG 5.5 freezing solution.
|
|
|
KEYWORD
|
|
|
Bovine embryos, EG 5.5, EM grid, OPS, Cryo-loop
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|
|
|