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KMID : 0900920020260030229
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Korean journal of Animal Reproduction
2002 Volume.26 No. 3 p.229 ~ p.234
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Studies on the Number of Sperm Penetrated Oocytes and Survival Rate of Frozen-thawed Epididymal Dog Sperm
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Kim Sang-Keun
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Abstract
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This study was carried out to investigate the general characteristics such as concentration sperm motility and abnormality of sperm on the whole epididymal semen(EWS), RSP-S(removed seminal plasma by saline) and RSP-T(removed seminal plasma by tris-buffer) semen and survival rates after freezing on motility of whole and RSP-S and RSP-T semen and extender containing 2~8% glycerol, and ability of frozen-thawed sperm to penetrate homologous oocytes. 1. The concentration, motility and abnormality of epididymal WES, RSP-S and RSP-T sperm were 4.25 pm 0.25(times10^{6} Cells/mell), 3.85pm0.20(times10^{6} Cells/mell), 4.05 pm 0.28(times10^{6} Cells/mell), 50.55 pm 2.75%, 67.25 pm 2.55%, 78.75 ms 3.55 and 9.45 pm 2.25%, 37.75 pm 2.10%, 24.25 pm 1.55%, respectively. 2. The survival rates of slow and rapid frozen epididymal RSP-S and RSP-T sperm were 35.00 pm 2.35%, 45.50 pm 2.15％ and 16.50 pm 3.55%, 22.55 pm 3.95%, respectively. The survival rate of epididymal WES and RSP-T sperm after freezing following dilution with tris-buffer containing 2~8% glycerol were 9.25 pm 1.55%~17.50 pm 2.50%. 3. The percentage of capacitated and acrosome-reacted sperm prier to culture for fresh and frozen -thawed epididymal RSP-T semen were 45.25 pm 5.75%, 7.06 pm 0.25%, 48.20 pm 6.80% and 13.00 pm 2.35%, 3.55 pm 0.85%, 15.50 pm 1.90%, respectively. The penetration rate the number of sperm per penetrated for fresh and frozen-thawed epididymal RSP-T sperm were 39.25 pm 4.72%, 34.24 pm 3.93% and 1.30 pm 0.33, 1.10 pm 0.50., respectively.
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KEYWORD
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Epididymal sperm, RSP-S, RPS-T, Freezing, Survival rate
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