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KMID : 0900920030270010009
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Korean journal of Animal Reproduction
2003 Volume.27 No. 1 p.9 ~ p.14
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Stable Transmission and Expression of TPO Transgene up to 10 Generation in the Transgenic Mice
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Chung Jin-Woo
Han Yong-Mahn
Lee Kyung-Kwang
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Abstract
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The pBT-L transgenic mice carrying human TPO gene in conjunction with bovine beta-casein promoter express human TPO in milk during lactation. In this study, stability of germ line transmission and expression of pBT-L transgene integrated into host chromosome were monitored up to generation F10 of transgenic pBT-L/15 line. When male mouse of generation F8 was crossbred with normal females, approximately half of offsprings (51.3pm18.98%) were identified as transgenic mice. Generation F9 and F 10 mice also showed similar transmission rates (43.8pm18.98% and 71.4pm26.98%, respectively), implying that pBT-L transgene can be transmitted stably up to long term generation in the transgenic mice. Expression levels of human TPO from milk of generation F9 and F10 mice were 1.1pm0.33 mg/ml and 1.1 pm0.45 mg/ml, respectively, which are similar to expression level of generation F2 mice. In conclusion, our results suggest that transgenic animals once established will continuously pass their transgenes to the progeny through the breeding program with the same productivity of human protein in their milk.
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