KMID : 0903520050480040425
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology 2005 Volume.48 No. 4 p.425 ~ p.430
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Production of ¥â-sitosterol by Cell Suspension Culture of Chrysanthemum coronarium L.
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Kim Hyun-Chul
Chung Ha-Young Lee So-Yeon Chung Ho-Yong Kim You-Jung Baek Nam-In Kim Sung-Hoon Choi Geun-Won Kim Dae-Keun Kwon Byoung-Mog Park Mi-Hyun Chung In-Sik
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Abstract
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¥â-sitosterol is a plant sterol that reduces cholesterol levels and inhibits the growth of human prostate and colon cancer cells. Optimal conditions for ¥â-sitosterol production were examined from cell suspension cultures of Chrysanthemum coronarium L. The callus induction was optimal in MS medium containing 1 mg/l NAA and 1 mg/l BAP. Cell suspension culture was also established from the callus. Optimal ¥â-sitosterol production was obtained when the cells were cultured at an initial density of 2 mg DCW/l in MS medium containing 1 X sucrose (30 mg/l), 1 X nitrogen (1900 mg/l KNO3, 1650 mg/l NH4NO3), and 1 X phosphate source (170 mg/l). In cell suspension cultures of C. coronarium L. using shake flasks, the peak content of ¥â-sitosterol was 150 ¥ìg/g DCW. In cell suspension cultures of C. coronarium L. using an air-lift bioreactor, the maximum ¥â-sitosterol content of 143.8 ¥ìg/g DCW was obtained at an air-flow rate of 100 cc/min.
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KEYWORD
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¥â-sitosterol, callus, suspension culture, air-lift bioreactor
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