Reactive oxygen species (ROS) have been implicated in the pathogenesis of various diseases, and play an important role in the skeletal muscle damage through strenuous exercise or its aging. To investigate whether H©üO©ü, as main precursor of highly reactive free radicals, induces apoptosis in mouse skeletal muscle cells C2C12, 3-(4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide(MTT) assay, terminal deoxynuclotidyl transferase -mediated dUTP nick end-labeling (TUNEL) assay, 4,6-diamidino-2- phenylindole (DAPI) staining, DNA fragmentation assay, reverse transcription-polymerase chain reaction (RT-PCR) and Western blot analysis were performed. Through morphological and biochemical analyses, it was demonstrated that C2C12 cells treated with H©üO©ü exhibit classical apoptotic features. In addition, it was shown that H©üO©ü induces increases in the levels of bax, and a decrease in the bcl-2 expression. Based on the results, H©üO©ü appears to activate specific intracellular death-related pathways and the induction of apoptosis in skeletal muscle cells.
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