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KMID : 1004820070080010059
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Journal of Biomedical Research
2007 Volume.8 No. 1 p.59 ~ p.68
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Effect of Lipids Containing Conjugated Linoleic Acids on Proliferation and Differentiation of Preadipocytic Cells
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Hue Jin-Joo
Lee Yea-Eun
Jeong Jae-Hwang
Lee Sang-Hwa
Lee Young-Ho
Hong Soon-Ki
Jeong Seong-Woon
Nam Sang-Yoon
Yun Young-Won
Lee Beom-Jun
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Abstract
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Conjugated linoleic acid (CLA) have been recently reported to have an anti-obesity effect in animals and humans. The objectives of this study were to investigate the anti-obesity effect of lipids containing CLA in 3T3-L1 preadipocytes. Cell viability was determined using WST-8 analysis and cell differentiation was determined by glycerol-3-phophate dehydrogenase (GPDH) activity. Lipid accumulation in differentiating 3T3-L1 cells was measured by Oil red O staining There were six experimental groups including vehicle control (DMSO), diglyceride (DG), CLA, triglyceride (TG)-CLA, DG+CLA20, and DG+CLA60. The mean viability of 3T3-L1 cells by treatments of test solutions at the concentrations of 10 to 1000 ㎍/ml was reduced in a dose-dependent manner. The test solutions showed a similar pattern in reduction of cell viability. Treatments of the test solutions at the concentrations of 500 μg/ml for 48 h in 3T3-L1 cells inhibited the differentiation of as measured by GPDH activity after incubation in adequate differentiation media for 8 days. In addition, lipid accumulation was also reduced as incubated for 8 days in differentiation medium after treatments of test solutions for 48 h in 3T3-L1 cells. Among them DG+CLA60 was the most effective in the inhibition of differentiation and lipid accumulation. These results suggest that the compounds including CLA as fatty acids may have respectable anti-obesity effects, regardless ofdiglyceride and/or triglyceride or CLA alone and that they may give a benefit for controlling overweight in humans.
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KEYWORD
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Conjugated linoleic acid, Diglyceride, Glycerol-3-phosphate dehydrogenase, Obesity, 3T3-L1 cells
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