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KMID : 1007519990080050338
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Food Science and Biotechnology
1999 Volume.8 No. 5 p.338 ~ p.340
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Liquefaction Methods for Production of Sulfated Mucopolysaccharides from Ciona intestinalis
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Kim, In Soo
PARK, Douck Choun/KIM, Seon Bong/Lee, Seung Han/Park, Jae Hong/Gu, Yeun Suk/Ji, Cheong Il
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Abstract
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The effect of liquefaction methods on the content of sulfated mucopolysaccharides (MPS) in Ciona intestinalis was studied. The extracts by pressure cooker at 121℃ and 1.5 ㎏/㎠ had the highest content of sulfated MPS (8.6-10.3%), followed by those treated with boiling water (4.3-5.9%). Acid/alkali and enzyme treatments produced lower contents of sulfated MPS than extracts by the pressure cooker. Treatments of Alcalase and Neutrase gave higher contents of sulfated MPS (5.4% and 3.7%, respectively) than other enzyme treatments. Compared with all liquefaction methods, pressure cooking for 1 hr was effective for the preparation of sulfated MPS products, and its recovery of sulfated MPS was approximately 50%.
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