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KMID : 1007520080170030623
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Food Science and Biotechnology
2008 Volume.17 No. 3 p.623 ~ p.630
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Determination of Aflatoxin B1 in Rice, Barley, and Feed by Noninstrumental Immunochromatographic Strip-test and High Sensitive ELISA
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Shim Won-Bo
Kim Jung-Sook
Kim Ji-Young
Choi JIn-Gil
Je Jung-Hyun
Kuzmina Nina Sergeevna
Eremin Sergei Alexandrovich
Chung Duck-Hwa
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Abstract
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A non-instrumental immunochromatographic (ICG) strip-test and direct competitive enzyme-linked immunosorbent assay (DC-ELISA) for aflatoxin B1 (AFB1) determination were developed and optimized. The detection limits of ICG striptest and DC-ELISA were 0.5 and 0.004 ng/mL, respectively, and these methods possessed a cross-reaction to aflatoxins. The results of spiked samples by both methods were coincided with the amount spiked AFB1 and the comparative analyses of 172 real samples by 2 immunoassays and high performance liquid chromatography (HPLC) showed a good agreement. Especially, the ICG strip-test is easier to perform and quicker, but less sensitivity than DC-ELISA. Both methods could analyze a high sample throughput with short time, but the sample throughput of ICG strip-test was better. Therefore, the ICG strip-test can be used as a simple, easy, non-instrumental, and fast screening technique for AFB1 determination.
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KEYWORD
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aflatoxin B1, mycotoxin, non-instrumental assay, immunochromatographic strip-test, ICG, enzyme-Linked immunosorbent assay, ELISA
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