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KMID : 1007520090180051273
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Food Science and Biotechnology
2009 Volume.18 No. 5 p.1273 ~ p.1278
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Allergenicity Assessment of Cry Proteins in Insect-resistant Genetically Modified Maize Bt11, MON810, and MON863
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Kim Jae-Hwan
Seo Young-Ju
Kim Ji-Young
Han Young-Shin
Lee Kwang-Shin
Kim Sun-Ah
Kim Han-Na
Ahn Kang-Mo
Lee Sang-II
Kim Hae-Yeong
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Abstract
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This study aimed to evaluate the potential allergenicity of Cry proteins in insect-resistant genetically modified (GM) maizes (Bt11, MON810, and MON863) using serum screening tests. Serum samples were obtained from Korean children (0-15 years old) with allergic symptoms who had positive maize-specific IgE. The levels of serum specific IgE was measured by the Phadia ImmunoCAP system and considered as positive when they are 0.35 kU/L or higher. Cry proteins (Cry1Ab in Bt11, mCry1Ab in MON810, and Cry3Bb1 in MON863) were expressed in Escherichia coli and purified for serum screening. The reactivity of purified Cry proteins was confirmed by IgE immunoblots in 50 patients (maize-sensitized patients). There was no reaction between Cry proteins and sera from maize-sensitized patients. Our results suggest that these Cry proteins are not likely to cause allergic reactions. Further studies using more sera from patients with true clinical allergies are needed to evaluate the potential allergenicity of novel proteins in GM maize.
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KEYWORD
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genetically modified maize, Cry protein, serum, allergenicity, immunoblot
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