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KMID : 1007520090180061470
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Food Science and Biotechnology
2009 Volume.18 No. 6 p.1470 ~ p.1475
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Comparison of Anthocyanin Content in Seed Coats of Black Soybean [Glycine max(L.) Merr.] Cultivars Using Liquid Chromatography Coupled to Tandem Mass Spectrometry
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Shin Sung-Chul
Lee Soo-Jung
Lee Sung-Joong
Chung Jong-Il
Bae Dong-Won
Kim Soo-Taek
Sung Nak-Ju
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Abstract
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The seed coat of the black soybean contains 3 main anthocyanins such as delphinidin-3-O-β-glucoside, cyanidin-3-O-β-glucoside, and petunidin-3-O-β-glucoside. As a part of our effort on discovering and breeding new black soybean cultivars which possesses specific anthocyanin component rich, we determined the anthocyanin profiles of the 2 cultivars recently developed soybean cv. Gaechuck #1 and cv. Gyeongsang #1, using liquid chromatography-tandem mass spectrometry (LC-MS/MS) and compared their content and identity with those of previously known 10 cultivar controls. The Cosmosil- 5C18-AR-II column were selected for the analysis because of the best peak separation. The column temperature was set up at 35oC. The mobile phase consisting of water containing 0.5%(v/v) formic acid and methanol gave good separation between the 3 anthocyanin analytes and internal standard (quercetin 3-O-β-rutinoside) and peaks with suppressed tail. The MS/MS spectra of each individual anthocyanin standard were detected in positive electron spray ionization (ESI) modes. It was disclosed that the anthocyanin contents of the soybean cv. Gaechuck#1 and cv. Gyeongsang#1 are roughly higher than those of the 10 controls.
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KEYWORD
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black soybean, anthocyanin, liquid chromatography, tandem mass spectrometry, plant polyphenol
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