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KMID : 1007520100190010185
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Food Science and Biotechnology
2010 Volume.19 No. 1 p.185 ~ p.191
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Essential Oil and 1,8-Cineole from Artemisia lavandulaefolia Induces Apoptosis in KB Cells via Mitochondrial Stress and Caspase Activation
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Cha Jeong-Dan
Kim Youn-Hwa
Kim Ji-Young
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Abstract
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Artemisia lavandulaefolia has been utilized in traditional medicines for the treatment of several diseases. In this study, we attempted to determine whether the essential oil and 1,8-cineole of A. lavandulaefolia induce apoptosis in KB cells. The oil and 1,8-cineole induced the cell death of KB cells as evidenced by the increased cell population in the sub-G1 phase, the appearance of condensed and/or fragmented nuclei, and the generation of a cleaved PARP product. The treatment of the cells with the oil also induced changes in the mitochondrial level of Bcl-2 and Bax, thereby inducing the release of cytochrome c into the cytosol. Furthermore, the oil increased the phosphorylation of extracellular signal-regulated kinase (ERK), c-jun N-terminal kinase, and p38 MAPK, but 1,8- cineole activated pp38 and pJNK at the same concentrations. These findings show that the mitochondrial and MAPKs pathways might be involved in the oil-induced apoptosis, and may enhance our current understanding of the anticancer functions of the oil to a greater degree than 1,8- cineole.
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KEYWORD
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Artemisia lavandulaefolia, essential oil, 1, 8- cineole, apoptosis, mitochondrial stress, MAPK, caspases
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