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KMID : 1007520100190051277
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Food Science and Biotechnology
2010 Volume.19 No. 5 p.1277 ~ p.1282
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Ginsenoside 20(R)-Rg3 Stimulates Glucose Uptake in C2C12 Myotubes via CaMKK-AMPK Pathways
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Kim Do-Yeon
Yuan Hai-Dan
Huang Bo
Quan Hai-Yan
Chung Sung-Hyun
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Abstract
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Based upon preliminary study showing stimulation of glucose uptake by 20(R)-Rg3, a stereoisomer of 20(S)- Rg3 ginsenoside found in red ginseng, we investigated the action mechanism of 20(R)-Rg3 in differentiated C2C12 myotubes. It was found that 20(R)-Rg3 stimulated translocation of glucose transporter 4 (GLUT4) and glucose uptake in a concentration-dependent manner. In addition, 20(R)-Rg3 phosphorylated AMP-activated protein kinase (AMPK) and Ca2+/Calmodulin-dependent protein kinase kinase (CaMKK) in time- and concentration-dependent manners. In the meantime, 20(R)-Rg3-induced glucose uptake and translocation of GLUT4 were attenuated in the presence of compound C (an AMPK inhibitor) or STO-609 (a CaMKK inhibitor). Taken together, this study is the first to demonstrate that 20(R)-Rg3 stimulated the glucose uptake via translocation of GLUT4 and this effect was seemed to be mediated by CaMKK-AMPK pathways in C2C12 myotubes.
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KEYWORD
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20(R)-ginsenoside Rg3, glucose uptake, C2C12 myotube, AMP-activated protein kinase, Ca2+/Calmodulindependent protein kinase kinase
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