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KMID : 1007520150240020658
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Food Science and Biotechnology
2015 Volume.24 No. 2 p.658 ~ p.664
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Chemical Characterization and Antitumor Activity of an Exopolysaccharide from Pholiota Squarrosa Quel. AS 5.245
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Zhao Haizhen
Wang Juan
Lv Fengxia
Bie Xiaomei
Lu Zhaoxin
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Abstract
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A water-soluble exopolysaccharide (PESP-1) was extracted and purified using DEAE Sephadex A-50 and Sephadex G-100 columns from a submerged culture broth of Pholiota squarrosa Quel. AS 5.245. PEPS-1 was investigated for antitumor activity against Heps tumors implanted in mice. An inhibition rate of 78.46% at a dosage of 100 mg/kg was observed. PEPS-1 significantly (p<0.05) increased the relative spleen/thymus indices of Heps tumor-bearing mice at a dosage of 100 mg/kg, compared with controls. Antitumor properties were probably related to stimulation of the immune response. Preliminary physicochemical analysis identified PEPS-1 as a heteropolysaccharide mainly containing D-mannose, D-glucose and D-galactose at molar ratios of 50:33:18. Small amounts of D-rhamnose and D-xylose were also detected. The average Mw of PEPS-1 was 3.26×104 Da. Structural features probably played an important role in the antitumor activity of PEPS-1.
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KEYWORD
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exopolysaccharide, Pholiota squarrosa, antitumor activity
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