KMID : 1007520170260010195
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Food Science and Biotechnology 2017 Volume.26 No. 1 p.195 ~ p.200
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In vitro anti-obesity effects of sesamol mediated by adenosine monophosphate-activated protein kinase and mitogenactivated protein kinase signaling in 3T3-L1 cells
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Go Geon
Sung Jung-Suk Jee Seung-Cheol Kim Min Jang Won-Hee Kang Kyu-Young Kim Dae-Young Lee Si-Hyoung Shin Han-Seung
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Abstract
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Sesamol is a phenol derivative of sesame oil and a potent anti-oxidant, anti-inflammatory, anti-hepatotoxic, and anti-aging compound. We investigated the effects of sesamol on the molecular mechanisms of adipogenesis in 3T3-L1 preadipocytes. The intracellular lipid accumulation accompanied by increased extracellular release of free glycerol was decreased during differentiation on treating 3T3-L1 with sesamol. Sesamol treatment on 3T3-L1 inhibited adipogenic differentiation by down-regulating adipogenesis-related factors (C/EBP¥á, PPAR¥ã, and SREBP-1). Lipid accumulation was repressed by decreasing fatty acid synthase and by up-regulating lipolysis-response genes (HSL and LPL). The molecular mechanisms of sesamol-induced inhibition in adipogenesis were mediated by increased levels of phosphorylated adenosine monophosphate-activated protein kinase and its substrate acetyl-CoA carboxylase. Sesamol treatment, in turn, modulated the different members of the mitogenactivated protein kinase family by suppressing phosphorylation of ERK 1/2 and JNK and by increasing the phosphorylation of p38. In summary, sesamol inhibits adipogenic differentiation by reducing phosphorylation levels of ERK 1/2 and JNK while inducing lipolysis by activating p38 and AMPK. Our results demonstrate that the molecular mechanisms of in vitro anti-obesity effects of sesamol are due to the combined effects of preventing both lipid accumulation and adipogenesis.
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KEYWORD
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sesamol, adipogenesis, anti-obesity, AMPK, lipolysis, MAPK
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