|
KMID : 1007520170260020507
|
|
Food Science and Biotechnology
2017 Volume.26 No. 2 p.507 ~ p.511
|
|
|
Monoolein, isolated from Ishige sinicola, inhibits lipopolysaccharide-induced inflammatory response by attenuating mitogen-activated protein kinase and NF-κB pathways
|
|
Ali Irshad
Manzoor Zahid
Koo Jung-Eun
Moon Seung-Ri
Byeon Sang-Hee
Yoo Eun-Sook
Kang Hee-Kyoung
Hyun Jin-Won
Lee Nam-Ho
Koh Young-Sang
|
|
|
Abstract
|
|
|
|
Ishige sinicola (I. sinicola) is an edible brown alga native to South Korea. In the present study, we screened the anti-inflammatory activity of monoolein isolated from I. sinicola. Monoolein pretreatment in lipopolysaccharide (LPS)-stimulated primary murine bone marrow-derived dendritic cells (BMDCs) showed strong dose-dependent inhibition of interleukin (IL)-12 p40, IL-6, and TNF-α cytokine production with IC50 values of 1.69±0.02, 6.87±0.37, and 5.19±0.56 μM, respectively. Pretreatment of monoolein attenuated the activation of MAPK and NF-κB pathways in the LPS-stimulated BMDCs by inhibiting the phosphorylation of p38, ERK1/2, JNK1/2, and IκBα. Furthermore, monoolein inhibited the production of NO and iNOS in RAW264.7 cells. Overall, our findings indicate that monoolein has a significant anti-inflammatory activity, and further studies regarding the potential of monoolein for medicinal use is warranted.
|
|
|
KEYWORD
|
|
|
Ishige sinicola, monoolein, pro-inflammatory cytokine, mitogen-activated protein kinase, NF-κB
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|
|