KMID : 1007520210300040545
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Food Science and Biotechnology 2021 Volume.30 No. 4 p.545 ~ p.553
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A facile macroporous resin-based method for separation of yellow and orange Monascus pigments
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Chen Suo
Su Dong-Xiao Gao Meng-Xiang Zhang Jia-Lan Liu Ying-Bao Wu Qing-Hua Yang Hua-Lin Li Li
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Abstract
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The yellow Monascus pigments (YMPs) named monascin and ankaflavin and the orange Monascus pigments (OMPs) named rubropunctatin and monascorubrin are two groups of bioactive components in a mixture state in the Monascus fermented products. In order to separate these two groups of bioactive pigments, a facile macroporous resin-based method was developed. The weak-polar resin CAD-40 was selected from the seven tested macroporous resins as it revealed better properties for the adsorption and desorption of the YMPs and OMPs. Then, CAD-40 resin was used for column-chromatographic separation. After eluted by 4 bed volumes of ethanol, the yellow group (monascin and ankaflavin) and the orange group (rubropunctatin and monascorubrin) were successfully separated and purified, with an increased content from 49.3% and 44.2% in the crude pigment extract to 85.2% and 83.0% in the final products, respectively. This method would be helpful for the large-scale separation and purification of Monascus pigment products with specific bioactivity.
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KEYWORD
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Monascus pigments, Macroporous resin, Separation, Purification
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