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KMID : 1007520210300040565
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Food Science and Biotechnology
2021 Volume.30 No. 4 p.565 ~ p.570
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Detection of GM Canola MS11, DP-073496-4, and MON88302 events using multiplex PCR coupled with capillary electrophoresis
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Lee Do-Geun
Park Ji-Eun
Kim Mi-Ju
Kim Hyun-Joong
Kim Hae-Yeong
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Abstract
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As of 2020, 11 GM canola events have been authorized as food for humans in Korea. However, there are no simultaneous multiplex detection methods for 3 GM canola events (DP-073496-4, MON88302, and MS11). Thus, we established the multiplex polymerase chain reaction (PCR) method coupled with capillary electrophoresis to detect 3 GM canola events. To verify the specificity of event-specific primers, various GM crops of 3 GM soybean events, 6 GM maize events, 2 GM cotton events and 11 GM canola events were prepared. The limit of detection of the developed multiplex PCR was approximately 0.0125% for 3 GM canola events. Certified GM canola and stacked events were analyzed to validate the developed multiplex PCR. This study focuses on establishing multiplex PCR coupled with capillary electrophoresis for newly approved GM canola events and contributes to efficient monitoring GM canola samples in Korea.
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KEYWORD
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Genetically modified canola, Event-specific detection, Multiplex polymerase chain reaction, Monitoring, Capillary electrophoresis
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