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KMID : 1007520230320060823
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Food Science and Biotechnology
2023 Volume.32 No. 6 p.823 ~ p.831
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Synergistic antimicrobial effect and mode of action of palmarosa oil-loaded nanoemulsion and citric acid against Pectobacterium carotovorum
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Shin Jeong-Yong
Kim Sang-Bin
Chang Yoon-Jee
Imm Jee-Young
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Abstract
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The synergistic antimicrobial activity of palmarosa oil (Cymbopogon martini, PO)-loaded nanoemulsion (PO-NE) and citric acid (CA) against Pectobacterium, the major pathogen for soft-rot disease, was evaluated. The combination of PO-NE and CA (PO-NE?+?CA) significantly improved the storage stability of PO-NE at 30 °C. Compared to the anti-Pectobacterium activity of alone, PO-NE?+?CA reduced the minimum inhibitory concentration (MIC) by 1/4 and 1/2, respectively. Bactericidal efficacy of PO-NE?+?CA against P. carotovorum PCC3 was similar of PO-NE alone in the MIC in time-kill kinetic assay. PO-NE treatment mainly influenced membrane integrity, while CA treatment strongly stimulated intracellular ATP depletion. This synergistic combination effectively reduced the use of PO-NE, imparting a strong flavor note without sacrificing the antimicrobial efficacy against Pectobacterium.
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KEYWORD
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Palmarosa oil nanoemulsion, Pectobacterium carotovorum subsp. carotovorum, Citric acid, Synergy, Soft-rot disease
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