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KMID : 1007520230320131851
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Food Science and Biotechnology
2023 Volume.32 No. 13 p.1851 ~ p.1860
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Optimization of ultrasound-assisted deep eutectic solvent extraction of bioactive compounds from pomegranate peel using response surface methodology
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Kim Hae-Jin
Yoon Kyung-Young
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Abstract
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The efficient extraction of polyphenols from pomegranate peels using a deep eutectic solvent (DES) and ultrasound-assisted extraction (UAE) was investigated. A Box?Behnken design was used to investigate the effects of four independent variables (water content, liquid-to-solid ratio, ultrasonic power, and extraction time) on total polyphenol content (TPC), punicalagin content (PC), and ellagic acid content (EC). Optimized DES-based UAE conditions were as follows: TPC (water content, 29.30%; liquid-to-solid ratio, 53.50 mL/g; ultrasonic power, 238.20 W; extraction time, 29.50 min), PC (water content, 25.65%; liquid-to-solid ratio, 44.20 mL/g; ultrasonic power, 120 W; extraction time, 20 min), and EC (water content, 33.13%; liquid-to-solid ratio, 60 mL/g; ultrasonic power, 300 W; extraction time, 20 min). Under these optimal conditions, the experimental values for TPC, PC, and EC were 67.50 mg GAE/g, 130.65 mg/g, and 2.04 mg/g, respectively; these values were consistent with the predicted values.
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KEYWORD
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Punicalagin, Ellagic acid, Deep eutectic solvent, Ultrasound-assisted extraction, Box?Behnken design
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