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KMID : 1023420100100050395
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Journal of Dental Hygiene Science
2010 Volume.10 No. 5 p.395 ~ p.401
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Morphology of Tooth and Smad4 Expression in NFI-C Deficient Mouse
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Bae Hyun-Sook
Kim Hye-Mi
Cho Young-Sik
Park Su-Jin
Choi Moon-Sil
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Abstract
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Over expression of TGF-β1 revealed the same phenotype as NFI-C deficient mouse. It has been reported that NFI-C deficient mice demonstrated abnormal odontoblast differentiation and aberrant dentin formation during root development. In the present study, in order to investigate the histological differences between wild type (WT) mouse and NFI-C deficient mouse, we compared morphological characteristics and smad4 expression between those mice. Hematoxyline-eosin (H-E) staining was used to investigate morphological changes and immunohistochemistry was also performed to observe the Smad4 expression pattern. In H-E staining, incisor of NFI-C deficient mouse showed an open area in the lingual root, irregular odontoblasts and osteodentin. Also, NFI-C deficient mouse showed short root and osteodentin in molar. In addition, Smad4 protein was strongly expressed in NFI-C deficient mouse compared with wild type. These findings suggest that NFI-C deficiency affects odontoblast differentiation and result in the formation of abnormal roots. Therefore, balancing between NFI-C and TGF-β signaling including Smad4 is important for the regulation of normal odontoblast differentiation and dentin formation.
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KEYWORD
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NFI-C, Smad4, Osteodentin, Tooth development
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