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KMID : 1023519960190010030
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Korean Journal of Veterinary Service
1996 Volume.19 No. 1 p.30 ~ p.38
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Detection of bovine rotavirus antigen by enzyme linked immunosorbent assay
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Ahn Jae-Moon
Yoo Ki-Jo
Lee Yong-Hee
Lee Jong-In
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Abstract
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Enzyme-linked immunosorbent assay (ELISA) was developed to detect rotavirus antigen in fecal samples using VP6-specific monoclonal antibody(2B12). The ELISA for rotavirus antigen detection found to have specificity to all bovine and porcine rotaviruses tested but not to bovine viral diarrhea virus and bovine coronavirus. The ELISA appeared to have similar sensitivity and specificity compared to fluorescence antibody assay(FA) and electropherotyping (PAGE).
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KEYWORD
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ELISA, rotavirus, fecal sample
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