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KMID : 1023519970200020195
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Korean Journal of Veterinary Service
1997 Volume.20 No. 2 p.195 ~ p.203
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Isolation of bovine Coronavirus from calves with diarrhea
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Ahn Jae-Moon
Yoo Ki-Jo
Lee Jong-In
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Abstract
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Fecal samples were collected from 47 calves with diarrhea and 12 clinically normal co-h-abitants, and tested for virus using MDBK cell cultures. Three cytopathic viruses were isolated from 8 fecal samples obtained from diarrheic calves. The isolated viruses were neutralized by bovine coronavirus hyperimmune serum In plaque reduction assay and were detected in the cytoplasm of MDBK cell by bovine coronavirus hyperimmune serum using immunofluorescence staining. The viruses agglutinated mouse erythrocytes only among the various animal erythrocytes tested and new isolates were identified as bovine coronavirus.
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KEYWORD
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Bovine Coronavirus, Calf diarrhea, Virus isolation
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