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KMID : 1023519970200040331
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Korean Journal of Veterinary Service
1997 Volume.20 No. 4 p.331 ~ p.338
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Diagnosis of bovine virus diarrhea-mucosal disease in breeding Holstein cows with diarrhea and vesicles
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Lee Seong-Hyo
Han Soo-Chol
Lee Joong-O
Yoon Yeo-Baik
Song Hee-Jong
Chai Hyo-Seok
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Abstract
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The purpose of this study was to establish early diagnosis for bovine virus diarrhea-mucosal disease(BVD-MD) Two Holstein among 22 breeding cows were shown ulcer in the mouth and watery diarrhea. Diarrheal feces and ulcerous lesion of the mouth from 2 cows were sampled for detection of viral antigen. BVD virus was isolated by inoculation of the samples to MDBK cells, and the cytopathic effects were observed in cultured MDBK cells which inoculated with virus isolates from the feces. Viral antigens were detected in the feces and ulceruous lesion by immunogold staining. The serum neutralization titers were shown 1 : 64 or greater in 8 blood samples by using BVD virus (NADL strain). By the RT-PCR, using reverse primer 5’-ACTCCATGTGCCATGTACAG-3’, forward primer 5’-ACTCCATGTGCCATGTACAG-3’, 285 base pair band specific to BVD virus was detected. In conclusions, the results of above tests which executed using the diarrheal feces and ulcerous lesion of the mouth and the isolates were conformed as BVD virus.
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KEYWORD
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Breeding Holstein cow, BVD-MD viral antigen, Immunogold staining, Cytopathic effect, RT-PCR
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