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KMID : 1023519970200040359
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Korean Journal of Veterinary Service
1997 Volume.20 No. 4 p.359 ~ p.370
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Studies on enterotoxigenic Escherchia coli isolated from piglets with diarrhea
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Kim Kwang-Jae
Yoon Kyo-Bok
Choi Bong-Chul
Shin Eun-Kyung
Kim Jong-Sool
Park Yong-Joo
Lee You-Sub
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Abstract
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This study was carried out to Investigate the biochemical characteristics, antibiotic susceptibility, serogroups and pili producibility test of enterotoxigenic Escherichia coli(ETEC) isolated from piglets with diarrhea in Kangwon province from March to October 1996. 1. Sixty eight E coli strains were isolated from 72 piglets with diarrhea and the biochemical and cultural reaction were compared with the classification criteria of Edwards and Ewing. 2. The serogroups of 26 isolates were classified as 08 : K87 6(8.8%), O20 : K1O1 4(5.9%), O141 : K85 4(5.9%), 09 : K103 : P987 3(4.4%), O45 : K 2(2.9%) 0139 : K82 2(2.9%), O64 : 2(2.9%), O149 : K91 1(1.5%), O157 : K88ac 1(1.5%) and O115 : 1(1.5%), respectively. 3. In antibiotic susceptibility test, the isolates showed high susceptible to Ak, Eno, Na, Gm, Am and Km, whereas resistance to Tc, Sm and Cf. 4. Sixty one strains(89.7%) of 68 I coli Isolates were resistant to one or more drugs. The isolates resistant to 2 and 3 or more drugs were 60.3% and 19.1%, respectively. Amog the 16 multiple resistant patterns, Sm Tc(11.5% ), Cf Sm Tc(11.5% ), Cf Cp Sm Su Tc(9.8% ) and Cf Cp Sm Su Tc(8.2%) patterns were frequently observed. 5. MRHA of guinea pig erythrocytes was detected in 9 out of 26 OK serotype and 9 out of 42 unidentified serotypes. MRHA titers of serotypes showed from 16 to 32 in O141 : K85 and no titers in O139 : K82. 6. By the GM1 ganglioside ELISA, -, -, and -hemolysin producing strains was detected as 36, 6, and 5 from heat labile enterotoxin(LT) of 47 ETEC, respectively. The distribution of LT toxin from 112 isolates was showed - hemolysin, 2 isolates -hemolysin and 3 isolates -hemol-ysin from 26 OK serotypes.
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KEYWORD
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Piglet with diarrhea, Enterotoxigenic Escherichia coli, Colibacillosis
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