KMID : 1023520010240040335
|
|
Korean Journal of Veterinary Service 2001 Volume.24 No. 4 p.335 ~ p.341
|
|
Rapid detection of Brachyspira hyodysenteriae in swine intestinal specimens by PCR
|
|
Suh Dong-Kyun
Do Yun-Jeong Ha Jong-Su Lee Kyeong-Hyeon Song Dong-Jun Lee Chun-Sik Bae Young-Chan Jung Suk-Chan Choi Won-Pil
|
|
Abstract
|
|
|
Swine dysentery caused by Brachyspira hyodysenteriae, an anaerobic, beta-hemolytic spirochete, is a severe mucohemorrhahic diarrheal disease that primarily affects pigs during the growing and finishing period. The current standard laboratory procedure to culture and identify B hyodysenteriae takes 3 to 7 days. This report present a rapid PCR for detection B hyodysenteriae in a single reaction using DNA from swine intestinal samples. The PCR produced a specific 421bp PCR product with template DNA purified from B hyodysenteriae, and the accuracy for detection of B hyodysenteriae by PCR results compared with those of conventional method was 100% in intestinal specimens. Nonspecific bands were not detected with B innocens, a nonpathogenic common inhabitant spirochete, including other enteric bacterial organisms. This procedure could detect as little as 50 pg of template DNA for B hyodysenteriae.
|
|
KEYWORD
|
|
Brachyspira hyodysenteriae, Swine dysentery, Diagnosis, PCR
|
|
FullTexts / Linksout information
|
|
|
|
Listed journal information
|
|
|