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KMID : 1023520020250030275
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Korean Journal of Veterinary Service
2002 Volume.25 No. 3 p.275 ~ p.283
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Molecular typing and detection of enterotoxin by multiplex PCR of Staphylococcus aureus isolated from bovine mastitis
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Kim Shin
Hong Hyon-Pyo
Kim Sang-Yun
Kwon Heon-Il
Lee Hee-Moo
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Abstract
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Forty strains of Staphylococcus aureus were isolated from mastitic milk. As a result of antimicrobial susceptibility test, the strains of S aureus revealed 47.5% were resistant to ampicillin and penicillin, and 7.5% to gentamicin. But 45% of isolates were sensitive to antimicrobial agents tested. In case of enterotoxin production, 56.3% of 16 strains produced enterotoxin D. Two strain of enterotoxin D producers produced both enterotoxin B and D. According to isolation date, 15 representative strains were selected. As a results of pulsed field gel eletrophoresis analysis of the 15 representative strains, 14 strains were identical. Therefore we consider the identical strains of S aureus have caused continuously bovine mastitis in this dairy farm. If autogenous vaccine can be made by the strains, it will work well for the prevention of bovine mastitis caused by S aureus.
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KEYWORD
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Staphylococcus aureus, Enterotoxin, Multiplex PCR, PFGE
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