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KMID : 1023520030260020105
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Korean Journal of Veterinary Service
2003 Volume.26 No. 2 p.105 ~ p.111
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Isolation and PCR detection of Listeria monocytogenes on raw beef and pork carcass
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Chae Hee-Sun
Kim Doo-Hwan
Kim Kyu-Hiun
Sin Bang-Woo
Jo Mi-Yoeng
Kweon Tek-Boo
Lee Jung-Hark
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Abstract
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From February 2000 to December 2001, A total of 1,785 samples was taken from beef and pork carcasses in Seoul. Seven(0.69%) Listeria spp. were isolated from the 1,014 of beef carcasses, and five(0.65%) were isolated from the 771 of pork carcasses. The isolates were identified L monocytogenes by API listeria, and VIDAS LMO kit, serological test and PCR assay were preformed. A total 12 strains of L monocytogenes were isolated form samples tested and all of the strains were classified into serotype 1. PCR primers are selected to amplify a 520-base pair(bp) DNA fragment from the listeolysin O gene(hlyA) of Listeria monocytogenes. A 520-bp product was detected in PCR with DNA from L monocytogenes, but not from the other Listeria species tested.
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KEYWORD
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L monocytogenese, PCR, Carcasses
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