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KMID : 1023520030260020121
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Korean Journal of Veterinary Service
2003 Volume.26 No. 2 p.121 ~ p.128
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Isolation and antimicrobial susceptibility test of Mannheimia haemolytica from slaughtered cattle
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Byun Jae-Won
Kim Kyoung-Ho
Lee Seung-Hwan
Lee Hye-Wha
Lee Jung-In
Hwang Hyun-Soon
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Abstract
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This study was carried out to identify and investigate antimicrobial susceptibility for Mannheimuia haemolytical which is responsible for shipping fever. Samples were collected from nasal and lung of 100 adult healthy cattle which are slaughtered in Samsung meat corporation located in Incheon metropolitan city. lung lesion index have been investigated within 0-5 range according to Shewen and Willkie(Can J Vet Res 52:30-36, 1988). Eighty-seven of 100 cattle were under normal condition with 0-1 ranges. A total of 129 strains were collected from blood and tryptic soy agar. Among these strains, 100 strains were identified with Staphylococcus, Streptococcus and enterobacteria containing E coli. Biochemical and fermentation assay of arabinose, trehalose, xylose, mannose, mannitol, lactose and salicin were tested to identify with Mannheimia sp. for 7 strains shown haemolytic activity on blood agar. Five strains were identified with Mannheimia haemolytica and 2 strains were untyped. In seasonal survey, Mannheimia sp recovered from fall to winter(5 of 7) have been highly isolated rather than those from spring to summer(2 of 7). Mannheimiz haemolytica were susceptible to antibacterials tested in this study but more resistant to oxytetracycline and streptomycin.
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KEYWORD
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Mannheimia haemolytica, Lung lesion index, Antibiotic susceptibility test
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