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KMID : 1023520040270010103
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Korean Journal of Veterinary Service
2004 Volume.27 No. 1 p.103 ~ p.109
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Identification of promoter sites in Babesia equi ema-l 5’ intergenic nucleotide: I. PCR amplification and restriction mapping
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Kwak Dong-Mi
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Abstract
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Babesia equi ema-1 5’ intergenic(IG) nucleotide was PCR amplified and analyzed for restriction sites in order to identify a promoter region in this IG nucleotide sequence. B equi ema-1 5’ IG specific primers identified a 1268 bp PCR product. The sequence had restriction sites for 34 restriction enzymes when analyzed by a computer program. Among them, 26 enzymes had only one restriction site, but the others had more than one sites. When four restriction enzymes (Bgll , HindⅢ, Kpn1 and BamH1) were treated to digest the 1268 bp nucleotide, they had restriction sites as expected by the computer program. Information of restriction sites in the 1268 bp IG nucleotide will be applied to select restriction enzymes for cloning the IG nucleotide to a vector.
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KEYWORD
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Babesia equi, Intergenic nucleotide, PCR, Restriction sites, Promoter
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