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KMID : 1023520090320030281
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Korean Journal of Veterinary Service
2009 Volume.32 No. 3 p.281 ~ p.286
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Determination and survey of fluoroquinolones in meats and eggs (II)
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Choi Yoon-Hwa
Kim Yoen-Joo
Lee Kyung-Hye
Kang Young-Il
Lee Jung-Hark
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Abstract
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Fluoroquinolones in muscle and egg were separated by liquid extraction and determined. The analysis was carried out using following conditions; C18 column (150×4.6mm, 5μm), mobile phase composed of D.W. (containing 0.4% triethylamine and phosphoric acid) : methanol : acetonitrile (780:100:120, v/v/v), quarternary pump at a flow rate of 0.9ml/min and 20μl of injection volume, fluorescence detector with EX 278nm/Em 456nm. The calibration range of seven fluoroquinolones showed linearity (r2?0.999) at concentration range of 0.025~0.8μg/ml. The recoveries in fortified muscle and egg represented more than 81.3%. The detection limits for ofloxacin, norfloxacin, ciprofloxacin, enrofloxacin, danofloxacin, saraloxacin and orbifloxacin were 3.1, 2.5, 3.6, 1.7, 0.9, 2.5 and 2.1μg/kg, respectively. We also monitored fluoroquinolones residue in the sample (chicken muscle 182, cattle muscle 140, pig muscle 139, egg 212) using EEC-plate (E. coli ATCC 11303) screening and HPLC confirmation methods. The screening test results, fluoroquinolones, antibacterial substances were all negative.
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KEYWORD
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Fluoroquinolone, Residue analysis, HPLC, HPLC-MSMS
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