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KMID : 1023520090320040335
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Korean Journal of Veterinary Service
2009 Volume.32 No. 4 p.335 ~ p.341
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Comparison of diagnostic methods for detection of Brucella species in dog blood samples
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Kwon Soon-Oh
Lam Truong-Wuang
Her Moon
Ahn Dong-Choon
Park Sang-Hee
Park Mi-Yeoun
Lee Young-Ju
Hahn Tae-Wook
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Abstract
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Canine brucellosis produce abortions and infertility in dogs and is currently diagnosed by serological methods such as rapid slide agglutination test with 2-mercaptoethanol (2-ME RSAT) and immunochromatographic assay (ICA). Bacterial isolation is considered gold standard for Brucella diagnosis and the polymerase chain reaction (PCR) is an alternative method to bacterial isolation. A total of 36 whole blood samples were collected from dogs reared in area of Chuncheon and were subjected to serology (2-ME RSAT and ICA for B. canis, Rose Bengal test and C-ELISA for B. abortus), blood culture and 3 types of PCRs (BSCP31, 16s rRNA, and OMP-2). All blood samples were negative by serology and blood cultures. The BCSP31 and the OMP-2 PCR detected 5 samples were positive whereas the 16S rRNA PCR detected all samples were negative as serological methods and blood culture did. From the results observed in the present study, we conclude that 16S rRNA PCR could be used for direct PCR for canine blood samples.
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KEYWORD
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Brucella, Dog, Blood sample, PCR, BCSP31, 16S rRNA, OMP-2
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