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KMID : 1023520100330010001
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Korean Journal of Veterinary Service
2010 Volume.33 No. 1 p.1 ~ p.6
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Monoclonal antibody-based enzyme immuno-slide assay (EISA) in the rapid diagnosis of Peste des petits ruminants of goats
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Das Kamol K.
Song Hee-Jong
Jang Hyung-Kwan
Shil N. K.
Rahman Md Siddique
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Abstract
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Monoclonal antibody (mAb)-based enzyme immune-slide assay (EISA) was used for the detection of Peste des petits ruminants (PPR) virus from field samples collected from a natural outbreak. The clinicopathological study was undertaken to diagnose the case primarily of PPR. Antigen was detected from discharges and faeces of infected goats and swabs of postmortem lesions prepared on glass slide or glass plate using acetone fixation. Nasal discharge collected at the early stage of disease course or lung is an appropriate ante- or postmortem sample for this technique, respectively. Convalescent polyclonal sera collected from recovered animals which were diagnosed as PPR by EISA showed high antibody titer against PPR by C-ELISA, demonstrating the satisfactory specificity of the test. Therefore, EISA is a sensitive and specific assay to confirm PPR infection both in field and laboratory conditions and especially suitable for developing country.
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KEYWORD
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Peste des petits ruminants (PPR), Goats, Enzyme immuno-slide assay
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