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KMID : 1023520120350020091
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Korean Journal of Veterinary Service
2012 Volume.35 No. 2 p.91 ~ p.97
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Seroprevalence of infectious bursal disease (IBD) and genetic sequence analysis of IBD virus
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Kang Mi-Seon
Chu Keum-Suk
Cho Hyun-Ung
Han Jae-Cheol
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Abstract
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The strategy for infectious bursal disease (IBD) control and its success rate under field conditions depends on hygiene management, IBD field pressure, level, and variation in maternally derived IBD antibodies. This study investigated the level of IBD-specific antibody by ELISA and the prevalence of IBD virus by PCR in broilers, white-semi broilers, and Korean native chickens raised in Jeongeup, Jeonbuk. IBD-specific maternally derived antibodies were measured from 698 chickens and the mean titers of maternal antibodies were 3,572±1,402 in broilers, 1,262±762 in white-semi broilers, and 1,932±912 in Korean native chickens. At 2 weeks after vaccination, the geometric mean antibody titers of broiler, white-semi broiler, and Korean native chicken were 582±427, 3255±1,080, and 1,023±499, respectively. According to sequence analysis of the variable virion protein 2 gene, 4 isolates were found to be very virulent IBDV, 9 isolates classical virulent, and 2 isolates intermediate plus vaccine strain.
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KEYWORD
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Infectious bursal disease, ELISA, PCR, Sequence analysis
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