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KMID : 1023520120350020105
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Korean Journal of Veterinary Service
2012 Volume.35 No. 2 p.105 ~ p.109
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Anti-apoptosis effects by Eimeria tenella infection in Madin-Darby bovine
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Lee Hyun-A
Hong Sun-Hwa
Kim Ok-Jin
Chung Yung-Ho
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Abstract
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Apoptosis is a host defense mechanism that the cell uses to limit production of infectious pathogens. Although many bacteria, viruses and parasites can induce apoptosis in infected cells, some pathogens usually exhibit the ability to suppress the induction of apoptosis in the infected cells. Sophisticated evasion strategies of obligate intracellular parasites, in particular prevention of host cell apoptosis, are necessary to ensure successful replication. To study the ability of Eimeria tenella in this regard, in vitro experiments were performed applying Madin-Darby bovine kidney (MDBK) cells as host cell. We have demonstrated that productive infection of adherent cell lines by E. tenella resulted in an anti-apototic effect. This phenomenon was confirmed using in situ terminal deoxynucleotidyl transferase- mediated (TdT) deoxyuridine triphosphates (dUTP)-fluorescein nick end labeling (TUNEL) assay to detect apoptosis. Therefore, E. tenella could complete its cycle of productive infection while inducing anti- apoptosis in the infected cells. This finding might have implications for the pathobiology of E. tenella and other Eimeria species.
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KEYWORD
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Protozoa, Apoptosis, Anti-apoptosis, Apicomplexa, Eimeria tenella
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