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KMID : 1023520120350040275
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Korean Journal of Veterinary Service
2012 Volume.35 No. 4 p.275 ~ p.281
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MLVA typing of Brucella canis isolated from dogs in Gyeongbuk province, Korea
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Kim Seong-Guk
Sohn Jun-Hyung
Jang Young-Sul
Kim Sang-Yoon
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Abstract
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Canine brucellosis is the zoonosis in worldwide and Brucella (B.) canis is a facultative intracellular pathogen that has a very limited host. MLVA-16 (Multilocus VNTR analysis) is a efficient method for genotyping of Brucella species. Various methods have been established for genotyping of Brucella species, but most of analytical method is lack reproducibility and limited capability to differentiate them. B. canis isolates (n=73) from 7 farms in Gyeongbuk province in 2003∼2010 were analyzed using 16 VNTR loci. Automatic electro-phoresis system was utilized for more high throughput and rapid simple discrimination. Thirty two genotypes were identified from 73 B. canis isolates. MLVA could contribute to molecular typing for epidemiological evaluation of canine brucellosis.
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KEYWORD
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Brucella canis, VNTR, MLVA, Genotype
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