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KMID : 1023520140370010001
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Korean Journal of Veterinary Service
2014 Volume.37 No. 1 p.1 ~ p.9
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Different immunological features of two genetically distinct type 2 porcine reproductive and respiratory syndrome, (PRRS)viruses
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Nadeem Shabir
Amina Khatun
Kim Won-Il
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Abstract
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Although it has been generally accepted that porcine reproductive and respiratory syndrome virus (PRRSV) induces weak and delayed protective immunity after infection, it is unclear that the same immunological features can be applicable to all PRRS viruses because huge genetic variation exists even among the same genotypes of PRRSV (Type 1 and 2). In the current study, two genetically distinct type 2 PRRSV strains (VR-2332 and JA142) which showed approximately 90% nucleotide homology based on ORF5 sequences were characterized by both in vitro and in vivo assessments to determine the immunological features of the viruses. For in vitro assessment, porcine alveolar macrophages (PAM) were infected with the viruses at 10.3 multiplicity of infection (MOI) and then supernatants and cells were collected separately at 36 hrs post infection to determine the relative expression levels of IL-1α,IL-12, TNF-α and INF-α/β by quantitative RT-PCR. In addition, five PRRSV-free pigs were inoculated with either of JA142 or VR2332 for in vivo assessment. Serum samples were collected every week until 6 weeks post challenge. The serum samples were analyzed for the levels of viremia, PRRSV nucleocapsid- specific antibody and virus neutralizing antibody. Based on those assessments, the two viruses showed different patterns of cytokine expression in PAM and immune responses in pigs after infection.These results indicate that genetically distinct PRRSV strains have different immunological features,which might be criteria for virus classification and selection of candidate virus strains for vaccine development in the future.
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KEYWORD
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PRRSV, Porcine alveolar macrophages, Cytokine expression, Serum virus neutralizing antibody, ELISA
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