|
KMID : 1023520150380030145
|
|
Korean Journal of Veterinary Service
2015 Volume.38 No. 3 p.145 ~ p.153
|
|
|
Visual detection of porcine circovirus 2 by loop-mediated isothermal amplification (LAMP) with hydroxynaphthol blue dye
|
|
Kong Ho-Chul
Kim Eun-Mi
Kim Hee-Jung
Kang Dae-Young
Yeo Sang-Geon
Park Choi-Kyu
Jeon Hyo-Sung
Kim Ji-Jung
Kim Young-Hwa
Park Jun-Cheol
Lee Chang-hee
Park Yu-Ri
|
|
|
Abstract
|
|
|
|
In this study, we developed a loop-mediated isothermal amplification (LAMP) with hydroxynaphtol blue dye (HNB) for rapid and direct visual detection of porcine circovirus 2 DNA with high sensitivity and specificity. The LAMP was completed in 40 min at 63oC, and the results of the LAMP can be confirmed by naked eye without any detection process. The sensitivity of the LAMP was 10-fold higher than that of the commercial PCR (cPCR) and real time PCR (rPCR) previously reported. In clinical application, the PCV2 detection rate of the LAMP was the same on porcine tissue samples (75.0%, 36/48) between porcine blood samples (75.0%, 39/52). The PCV2 detection rate (75.0%) of LAMP was higher than those of the cPCR and rPCR (67.3%, 35/52) in blood samples. In conclusion, the LAMP developed in the study could be an useful alternative method for the detection of PCV2 in the swine disease diagnostic laboratories.
|
|
|
KEYWORD
|
|
|
Porcine circovirus 2 (PCV2), loop-mediated isothermal amplification, LAMP, polymerase chain reaction, PCR, real time-PCR (rPCR)
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|