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KMID : 1023520160390020081
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Korean Journal of Veterinary Service
2016 Volume.39 No. 2 p.81 ~ p.86
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Experimental studies of homogeneity and stability honeybee venom using ultra-high performance liquid chromatography
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Han Sang-Mi
Kim Se-Gun
Hong In-Phyo
Woo Soon-Ok
Jang Hye-Ri
Lee Kyung-Woo
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Abstract
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Honeybee venom (BV) from Apis mellifera L. has been used as natural antimicrobial compounds in pigs, cows, dairy cattle and chicken farms in Korea. The purpose of this study was conducted to confirm homogeneity and stability of BV dissolved with distilled water or saline solution. Melittin was analyzed with ultra-high performance liquid chromatography (UPLC) for BV to secure the validation of analysis. BV at concentration of 1 mg/mL was dissolved with distilled water or saline solution at room temperature. Homogeneity of BV dissolved with distilled water or saline solution at upper, milddle, and lower layers all satisfied the accuracy and precision criteria. Stability of BV dissolved with distilled water or saline solution for 7 days all satisfied the criterion both light and dark storage condition. BV has satisfied with homogeneity and stability in distilled water or saline solution at room temperature under light or dark condition. The results of this study suggest that BV has a possibility as the substitute of natural antimicrobial agents for the animal drugs and feed additives.
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KEYWORD
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Honeybee venom, Melittin, UPLC, Homogeneity, Stability
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