|
KMID : 1023520160390030167
|
|
Korean Journal of Veterinary Service
2016 Volume.39 No. 3 p.167 ~ p.174
|
|
|
Analysis of low molecular organic compounds produced during the spoilage of dairy cattle compound feed
|
|
Kim Yong-Tak
Moon Hyung-In
Yi Kwon-Jung
Kim Gyeom-Heon
Kim Soo-Ki
Kim Dong-Woon
|
|
|
Abstract
|
|
|
|
In this study, we analyzed for the changes of low organic compounds during 4 weeks incubation though inoculation of harmful microorganisms on commercial feed. Two percent of overnight cultures of Exiguobacterium acetylicum, Acinetobacter calcoaceticus, Aspergillus flavus and Fusarium graminearum were inoculated in feed, respectively. After adjusting moisture level to 50% for the promotion of feed spoilage, pH was decreased to 4.58∼5.03 and microorganism was ranged to 6∼10 log10CFU/g. The compounds were compare between aflatoxin G1 producing feeds and aflatoxin G1 non-producing feeds. Aflatoxins G1 were detected by the immunoaffinity column clean-up method with HPLC-FLD, and were confirmed in samples incoulated by Aspergillus flavus and Acinetobacter calcoaceticus. Koiganal II, cy-clohexanol and butadien-one were detected from samples (the non-sterilized inoculated feed) by Aspergillus flavus and Acinetobacter calcoaceticus. Respectively as aflatoxin G1 pre-detected substance, Koiganal II, cyclohexanol and butadien-one may be useful substance for the pre-detection of aflatoxin G1.
|
|
|
KEYWORD
|
|
|
Aflatoxin, Koiganal II, Cyclohexanol, Butadien-one, low organic compounds
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|