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KMID : 1023520170400030161
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Korean Journal of Veterinary Service
2017 Volume.40 No. 3 p.161 ~ p.168
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Histopathological features and viral genome detection in caprine arthritis encephalitis virus infected dairy goats in Korea
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Son Ga-In
Cho Eun-Sang
Shin Hyun-Jin
Son Hwa-Young
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Abstract
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Caprine arthritis encephalitis (CAE) virus is a causative agent of caprine arthritis-encephalitis. In our previous study we reported a prevalence of CAE. In this study, we described the further detailed pathological features of CAE and examined the detection of virus by in situ hybridization (ISH). Histopathologi-cally, interstitial pneumonia and bronchopneumonia in lung, focal inflammation in mammary glands, perivascular cuffing in brain, arthritis, and focal necrosis, mild steatosis, inflammatory cell infiltration of liver were noted. CAEV proviral-DNA was identified by nested polymerase chain reaction (PCR) in blood cells, brain, synovial fluid, and lymph node. Confirmation by nested PCR involved amplification of a 296 bp (1st PCR) and 185 bp (2nd PCR) fragments corresponding to a conserved region on the gag gene of CAEV. Positive ISH signals were detected in the brain and liver. In conclusion, significant histopathological findings included parenchymal infection in various organs, including the lung, liver, brain, joint, and mammary gland were noted in the CAEV infected dairy goat. ISH can help confirm the diagnosis of CAE in formalin-fixed samples.
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KEYWORD
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Caprine arthritis encephalitis virus (CAEV), Histopathology, Nested polymerase chain reaction (PCR), In situ hybridization
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