Àá½Ã¸¸ ±â´Ù·Á ÁÖ¼¼¿ä. ·ÎµùÁßÀÔ´Ï´Ù.
KMID : 1023520170400030187
Korean Journal of Veterinary Service
2017 Volume.40 No. 3 p.187 ~ p.192
Production and characterization of monoclonal antibodies against an avian influenza virus (H9N2)
Lim Yong-Hwan

Phan Le Van
Mo In-Pil
Koo Bon-Sang
Choi Young-Ki
Lee Seung-Chul
Kang Shien-Young
Abstract
In this report, fifteen monoclonal antibodies (MAbs) against an avian influenza virus (H9N2 subtype) were newly produced and characterized. These MAbs proved to react to the epitopes of nucleocapsid protein (NP), hemagglutinin (HA), neuraminidase (NA) and non-structural protein 1 (NS1) of Korean H9N2 strain, respectively. Two HA-specific MAbs showed the ability to inhibit the hemagglutination activity of H9N2 subtype avian influenza virus when tested by hemagglutination inhibition (HI) assay. All MAbs did not cross-react with other avian-origin viruses (Newcastle disease virus, infectious bursal disease virus, infectious bronchitis virus and avian rotavirus) by immunofluorescence test or enzyme- linked immunosorbent assay. The MAbs produced in this study could be useful as the materials for diagnostics and therapeutics against Korean-lineage H9N2 virus infections.
KEYWORD
Avian influenza virus, H9N2, Monoclonal antibody
FullTexts / Linksout information
Listed journal information
ÇмúÁøÈïÀç´Ü(KCI)