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KMID : 1023520170400040253
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Korean Journal of Veterinary Service
2017 Volume.40 No. 4 p.253 ~ p.258
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Development of new analytical methods using high performance liquid chromatography for animal hormones; gonadorelin, progesterone, oxytocin and estradiol
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Jung Kyung-Hun
Jeong Mi-Young
Park Hae-Chul
Hossain Md Akil
Kim Dae-Gyun
Lee Kwang-Jick
Kang Jeong-Woo
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Abstract
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The aim of this study is to develop an optimal analytical method for gonadorelin, progesterone, oxytocin and estradiol, the major components of hormones. A relatively simple and reproducible method using high performance liquid chromatography was developed and as a result of the measurement of specificity, linearity, repeatability, accuracy and intermediated precision, the validity of the developed method was verified with the result of meeting the verification criteria of analytical method validation. Using this newly developed method, 12 post-market veterinary products were tested and the ingredient content were 91.9∼116.4%, which satisfied the 90∼120% condition of the administrative measure standard. Therefore, if the newly developed method is used for the collection examination of hormone in veterinary medicine, it can be useful as an approved test method.
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KEYWORD
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Gonadorelin, Progesterone, Oxytocin, Estradiol, HPLC
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