KMID : 1023520200430040261
|
|
Korean Journal of Veterinary Service 2020 Volume.43 No. 4 p.261 ~ p.265
|
|
Determination of cyromazine in commercial insecticides using HPLC-DAD
|
|
Kim Young-Wook
Han Bok-Hee Kang Young-Eun Rhee Chae-Hong Seo Sang-Ji Kim Soo-Hee Jeong Woo-Seog Her Moon
|
|
Abstract
|
|
|
Each commercial cyromazine insecticide has different HPLC conditions. The aim of this study was to establish a standardized chromatographic method for the determination of cyromazine in commercial insecticides. The separation was achieved on two C18 columns - Waters ¨Þ Bondapak C (4¡¿300 nm i.d., 10 ¥ìm) and X bridge (4.6¡¿250 nm i.d., 5 ¥ìm) using a mobile phase composed of water/methanol/ ethanolamine (76:24:0.1, v/v), with UV detection at wavelengths 230 nm and 254 nm. A total of six commercial cyromazine insecticides were analyzed. In this study, the optimal high-performance liquid chromatography conditions for the analysis of cyromazine were as follows: a mobile phase of water/ methanol/ethanolamine (76:24:0.1, v/v) at a flow rate of 1.0 mL/min and a detection wavelength of 230 nm using a X bridge C18 column (4.6¡¿250 nm i.d., 5 ¥ìm) at a column temperature of 25¡ÆC. The calibration curve was linear in the concentration range of 5¡50 ¥ìg/mL, with a correlation coefficient of 0.99995. The cyromazine detection limit was 0.2 ¥ìg/mL, and the limit of quantification was 0.59 ¥ìg/mL. The percentage recovery ranged from 99.8% to 101.0% for cyromazine, and the relative standard deviation was not over 2.0%. The cyromazine concentration ranged from 92.7% to 109.4% and was within the acceptable range (90¡120%) for the percent of the labeled amount. This method was found to be suitable for determining cyromazine in commercial insecticides.
|
|
KEYWORD
|
|
Cyromazine, Insecticide, HPLC, Validation
|
|
FullTexts / Linksout information
|
|
|
|
Listed journal information
|
|
|
|