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KMID : 1023520220450010039
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Korean Journal of Veterinary Service
2022 Volume.45 No. 1 p.39 ~ p.45
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Re-evaluation on conversion system of BactoScan and BactoCount for raw milk in South Korea
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Kim E-Seul
Kim Jin-Hwan
Byun Yeong-Seob
Park Da-Som
Kim Ha-Young
Yoon Soon-Seek
Moon Jin-san
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Abstract
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The total bacteria count is significantly important factor for hygienic quality in raw milk. BactoScan FMTM and BactoCount IBCTM are the automated instruments for the determination of the total bacte-rial count in raw milk. They have been used after calibration by standard plate count (SPC) method in South Korea since 2000. It is necessary to re-evaluate for total bacterial counter according to the improvement of milk quality and the change of milk quality grade. Therefore, this study was evalu-ated the conversion mode of the two machines by SPC method. We collected 921 bulk-tank milk samples throughout the concentration range of 1,000∼1,000,000 CFU/mL from June 2020 to Octo-ber 2021. As a result, when compared by the SPC value, there was a slight difference in total bacterial count in BactoScan below 10,000 CFU/mL and above 200,000 CFU/mL and in BactoCount above 100,000 CFU/mL, respectively. Therefore, the conversion factor for BactoScan and the conversion equation for BactoCount were newly adjusted based on SPC value, and then the correlation coef-ficients (R2) was 0.85 or higher. In addition, the correlation (R2) between BactoScan and BactoCount was 0.91, which means the results were high positive correlation. These results are expected to con-tribute to improving the accuracy of the automated instruments for determining of total bacterial count in raw milk.
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KEYWORD
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BactoCount, BactoScan, Conversion system, Raw milk, Total bacterial count
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