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KMID : 1023620060300030175
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Reproductive and Developmental Biology
2006 Volume.30 No. 3 p.175 ~ p.180
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Development of Somatic Cell Nuclear Transfer Bovine Embryos following Activation Time of Recipient Cytoplasm
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Park Sun-Young
Kwon Dae-Jin
Park Choon-Keun
Yang Boo-Keun
Cheong Hee-Tae
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Abstract
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This study was conducted to examine the effect of pre activation treatment and activation time of recipient cytoplasm on the development of bovine somatic cell nuclear transfer(NT) embryos. Donor cells were transferred and electrofused to enucleated oocytes before(pre-AC) or after activation(post-AC). Activation was induced with a combination of -ionophore(A23187) and DMAP. NT embryos were cultured in CR1aa containing 3 mg/ml BSA for 9 days. Some NT embryos were fixed at 0.5 to 2.5 hr after fusion(for post-AC) or activation(for pre-AC) for confocal microscopy. Developmental rate to the blastocyst stage was slightly high in the post-AC group(20.6%) compared to that of pre-AC group(15.3%). However, developmental speed of embryos in the pre-AC group was faster than that of embryos in the post-AC group. Development rates to the blastocyst stage were similar among different activation time before fusion(0.5,2 and 4 hr). The result of the present study suggests that development and nuclear morphology are affected f the activation status of the recipient cytoplasm before fusion.
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KEYWORD
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SCNT, Activation time, MPF activity, In vitro development, Bovine
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