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KMID : 1023620080320030159
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Reproductive and Developmental Biology
2008 Volume.32 No. 3 p.159 ~ p.166
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Generation of Transgenic Chickens that Produce Bioactive Human Thrombopoietin
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Kwon Mo-Seon
Koo Bon-Chul
Roh Ji-Yeol
Lee Hyun-Na
Kim Teo-An
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Abstract
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e report here the generation of transgenic chickens that produce human Thrombopoietin (hTPO) using replication-defective Moloney murine leukemia virus (MoMLV)-based vectors packaged with vesicular stomatitis virus G glycoprotein (VSV-G). For the retrovirus vectors, we used hCMV (human Cytomegalovirus) internal promoter to drive the hTPO gene. After confirming the expression of the hTPO gene in various target cells, the concentrated solution of recombinant retrovirus was injected beneath the blastoderm of non-incubated chicken embryos (stage X). The biological activity of the recombinant hTPO in target cell was significantly higher than its commercially available counterpart. Out of 132 injected eggs, 11 chicks hatched after 21 days of incubation and 4 hatched chicks were found to express vector-encoded hTPO gene. However, 3 out of the 4 transgenics died within one month of hatching. The major significance of this study is that it is one of the very few successful reports on the production of transgenic chickens as bioreactors aiming mass production of commercially valuable and biological active human cytokine proteins.
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KEYWORD
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Transgenic chicken, Human thrombopoietin (hTPO), Retrovirus vector, Bioreactor, Biological activity
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