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KMID : 1023620080320030199
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Reproductive and Developmental Biology
2008 Volume.32 No. 3 p.199 ~ p.203
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The Expression of Plasma Progesterone and Estradiol-17 Level before Parturition in the Recipients Pregnant by Hanwoo SCNT Embryos
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Hwang Seong-Soo
Choi Sun-Ho
Chang Yoo-Min
Ko Yeoung-Gyu
Yang Byong-Chul
Im Gi-Sun
Min Kwan-Sik
Seong Hwan-Hoo
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Abstract
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This study was performed to analyze the characterization of plasma hormonal levels during pregnancy in the Hanwoo recipients pregnant by artificial insemination (AI) or somatic cell nuclear transfer (SCNT) embryos. The synchronized recipients pregnant by SCNT embryos produced by Hanwoo fetal fibroblast cells (n=8) and by AI (control, n=5) were used. The plasma hormonal levels were measured by RIA (P4 and E2) and ELISA (cortisol), respectively. In control, the increase of E2 and the decrease of P4 were occurred immediately before the initiation of parturition. The expression pattern of plasma P4 was similar in both groups from 50 to 10 days before parturition, however, it did not decrease even at the expected date of labor in the SCNT recipients. The plasma cortisol was expressed a lower level during pregnancy in the SCNT recipients. But, the cortisol was increased in the cow aborted around 100 days of pregnancy (n=1). Based on these results, it can be postulated that the failure of the hormonal changes immediately before parturition in the SCNT recipients may be one of the most important reasons for a delayed parturition in clone calving.
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KEYWORD
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E2, P4, Cortisol, Initiation of parturition, SCNT
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