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KMID : 1023620080320040229
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Reproductive and Developmental Biology
2008 Volume.32 No. 4 p.229 ~ p.235
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Identification of Differentially Expressed Genes in the Dicer 1 Knock-down Mouse Embryos using Microarray
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Lee Jae-Dal
Cui Xiang-Shun
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Abstract
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Silencing of Dicer1 by siRNA did not inhibit development up to the blastocyst stage, but decreased expression of selected transcription factors, including Oct-4, Sox2 and Nanog, suggesting that Dicer1 gene expression is associated with differentiation processes at the blastocyst stage (Cui et al., 2007). In order to get insights into genes which may be linked with microRNA system, we compared gene expression profiles in Gapdh and Dicer1 siRNA-microinjected blastocysts using the Applied Biosystem microarray technology. Our data showed that 397 and 737 out of 16354 genes were up- and down-regulated, respectively, following siRNA microinjection (p<0.05), including 24 up- and 28 down-regulated transcription factors. Identification of genes that are preferentially expressed at particular Dicer1 knock down embryos provides insights into the complex gene regulatory networks that drive differentiation processes in embryos at blastocyst stage.
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KEYWORD
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Mouse, Dicer1, siRNA, Microarray
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